This book is developed using MATLAB software. It is useful for the beginner to start with simple steps and reaches to the advanced level. If you've not used GUI in MATLAB before, the first chapter will help you to get started. If you're already familiar with GUI in MATLAB, you can glance over the most of the concept from first chapter. This book is useful for the degree and post graduate courses to lean and get hands on expertise on GUI in MATLAB environment. GUI in MATLAB environment is the programmable and has the same way of designing GUI structure as that of other programming languages such as VB, .NET, Java etc. Thus it can be used to teach programming principals also. In most of the colleges MATLAB course has replaced the traditional GUI course and GUI in MATLAB is referred as principal computational tool used throughout the curriculum. This book is very much useful for engineering, mathematics and scientific communities. Many Degree and Post graduate colleges are require a course based entirely on GUI in MATLAB as interactive application development tool in the curriculum. Contents of this book can be used directly for one semester of engineering and science courses.